Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts
Abstract Background Xenograft samples used to test anti-cancer drug efficacies and toxicities in vivo contain an unknown mix of mouse and human cells.Evaluation of drug activity can be confounded by samples containing large amounts of here contaminating mouse tissue.We have developed a real-time quantitative polymerase chain reaction (qPCR) assay u